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Dr. Jacqueline Stevens

Faculty Manager

Ph. (601) 979-3462

jacqueline.j.stevens@jsums.edu

 

Ms. Alice Walker

Lab Technician

Ph: (601) 979-4333

alice.m.walker@jsums.edu


Facility Overview

Today, biomedical researchers require advanced technology such as genomics and gene expression, proteomics, bioinformatics, translational research, and biological imaging. The MCB Core Laboratory is instrumental in facilitating biomedical research in many of these areas.  This facility has provided the framework to nurture biomedical research and career development opportunities for new and established investigators in molecular and cellular research at JSU. Using recombinant DNA technology, the MCB Core Laboratory has enhanced the research capabilities of investigators at JSU by making available a variety of instrumentation and techniques at the cellular and molecular levels. The MCB Core Laboratory provides investigators with instrumentation access and tools to conduct cutting edge biomedical research. 

Specific Aims

The specific aims of the MCB Core Laboratory are:

  • To provide a variety of services and technical support in molecular biology and DNA technology  by offering equipment access, expert advice, demonstration of techniques, operation of equipment, participation in discussion and data interpretation, and hands-on-training in molecular biology and cell culture;

  • To increase faculty training in recombinant DNA technology to strengthen faculty development and technology transfer.

  • To provide a research environment and instrumentation for investigators to pursue biomedical research opportunities and enhance overall research productivity; and

  • To foster collaborative research relationships among RCMI investigators and other MCB core users at JSU and other research intensive institutions. 

Research Support Services of MCB Core Laboratory:

a. Genomics

  • Nucleic Acids isolation and purification

  •  Hybridization analysis of DNA/RNA

  • Gel electrophoresis

  • Southern and Northern blotting

  • Gene Expression and Detection

  • RT-PCR

  • Fluorescent Differential display-RT-PCR (FDDRT-PCR)

  • Genetic Manipulations

  • Subcloning

  • Sequencing

  • Plasmid DNA isolation and purification

  • Gene Transformation

b.   Proteomics 

  • Protein Isolation

  • Blotting and Hybridization

  • Western Blotting

  • Immunoblotting and Immunoprecipitation

  • Protein Determination Assays

  • Microsome preparation

  •  Amino acid analysis

  • Column Chromatography (CM, DEAE and HPLC)

  • Enzyme-Linked Immunosorbent Assay (ELISA)

c.   Functional Assays 

  • Cytotoxicity Assay (MTT)

  • [3H] Thymidine Incorporation Assay (non radiation)

  • Trypan blue exclusion Assay

  • Lipid Peroxidation Assay

  • Apoptotic DNA Laddering Assay

  • Comet Single Cell Assay

  • Flow Cytometry Analysis (FAC)

  • Annexin-V Assay

  • Caspase 3 Assay

  • MAP Kinase Assay

  • Akt Assay

  • cAMP Assay

d.   Cell Culture 

  • Subculture cells

  • Cryopreservation of cell lines

  • Viability Assay

  • Assist in morphological characterization of cell cultures by microscopic analysis

  • Consultation to investigators regarding techniques prior to experimentation

e.   Other Technical Assistance

  • Measurement of stress-related enzymes (TNF alpha, GM-CFS and IL1β)

  • Assist in preparation of tissue samples for Scanning and Transmission electron microscopy

Resources and Environment

The MCB Core Laboratory is located in the John A. Peoples (JAP) Building in Rooms 416 and 419 with dimensions of 30 sq ft by 31 sq ft and 14.5 sq ft by 3.5 sq ft respectively. The current resources in MCB Core Laboratory include:   

  • Automatic Environmental Speed Vac System (Savant)

  • Freeze Drying System with glassware (Savant)

  • Cryosafe Liquid Nitrogen Tank (Forma Scientific)

  • Nucleovision 760 Imaging Workstation (Gel Documentation System & Computer) (Nucleotech)

  • Nucleic Acid Sequencing System & Accessories (Bio Rad) Mastercycler Gradient Thermocycler (Eppendorp Scientific)

  • Kodak X-M35 Automatic Processor and Densitometer (Mid-South)

  • Biosafety Cabinet with UV (Forma Scientific)

  • Stratalinker 1800 UV Crosslinker (Stratagene)

  • Gel Dryer (Bio Rad)

  • 2 Power Plus Upright Freezers (Forma Scientific)

  • Water Baths (VWR)

  • UV Spectrophotometer (Varian)

  • UV Spectrophotometer (BioRad)

  • Refrigerator (4ºC glass door) (Fisher)

  • Microwave (Goldstar)

  • Incubator/oven (Napco)

  • Ice Maker (VWR)

  • Tissue Homogenizer (Omni)

  • Horizontal Electrophoresis Apparatus (BioRad)

  • Vertical Electrophoresis Apparatus (Amersham)

  • ElectroBlotting System -Transfer (Amersham)

  • Olympus Inverted Phase Contrast Microscope with Camera (C-Squared)

  • Analytical Balance (Fisher)

  • Refrigerated Microcentrifuge 4ºC (Forma Scientific)

  • pH Meter (Fisher)

  • Precision 37ºC Incubator (Fisher)

  • Humidified CO2 Incubators (Sanyo)

  • Sonifer Cell Disruptor Model 250/450 (VWR Scientific)

  • Olympus Epifluorescence Microscope Nikon E-600 Imaging System with Camera ©-Squared)

  • Water Purification System (Barnstead)

  • CEQ 8000 Genetic Analysis System (Beckman Coulter)